Fig. 5.

Neutrophils are the predominant peritoneal cellular subset that express pro-IL-1β⁺ at early time points following P. aeruginosa infection. C57BL/6 (n = 15) and ASC^−/− (n = 15) mice were infected intraperitoneally with a sublethal dose (3 × 10⁶ CFU) of WT PA14. Mice were euthanized 4 h postinfection and peritoneal lavage was collected. A: representative dot plots depicting the gating scheme for pro-IL-1β⁺ cells at 4 h postinfection. Bacteria were excluded by gating. B: percentage of pro-IL-1β⁺Ly6G⁺ (left) and pro-IL-1β⁺F4/80⁺ (right) cells out of the total peritoneal cells at 4 h postinfection. C: percentage of Ly6G⁺F4/80⁻ (left) and Ly6G⁻F4/80⁺ (right) within the gated CD45⁺pro-IL-1β⁺ population as depicted in A above. Data are expressed as means ± SD from 5 independent biological experiments.