Figure 2. Energy funneling efficiency (ε) determination in the single funnel approximation.

(A) Representative example of a polarization portrait decomposition. The polarization portrait is presented as a linear combination of two components: ‘Independent Chromophores’ and ‘Single Funnel’. In the former (B) the excited and emitting chromophores are the same, while in the latter (C) the absorbing states transfer their energy to a single pool of emissive states, (EET-emitter), which emits fluorescence. The parameter ε determines the contribution of the ‘Single Funnel’ component to the whole signal. θ_ex is the main orientation of the dipoles responsible for fluorescence excitation. θ_f is the main orientation of the EET-emitter.