Table 3. Precision, accuracy, LOD, and LOQ.

Matrix		HCAs													AA
		Conc. AA (ng/g)	MeIQ	IQ	Glu-P-1	Glu-P-2	MeIQx	Norhrman	Harman	Trp-P-2	PhIP	Trp-P-1	AaC	MeAaC
Non-fat liquid	Intra-day precision (%)	0.67	3.7	0.9	8.8	11.2	7.2	6.0	2.8	5.9	13.3	8.3	10.8	-	2.9
		3.33	4.8	2.4	8.5	5.9	4.0	2.3	1.8	10.9	10.8	9.5	10.5	9.6	2.9
		6.67	1.6	1.7	4.9	5.1	3.4	3.0	1.8	5.8	8.9	7.6	7.5	2.0	2.4
	Accuracy (%)	0.67	107.1	109.4	95.9	106.5	114.7	72.7	73.7	104.4	110.1	99.8	117.4	-	105.3
		3.33	97.0	108.9	106.8	109.9	103.2	104.6	98.1	114.5	108.2	104.8	92.6	95.5	100.1
		6.67	103.6	108.9	84.9	93.7	100.9	101.9	100.9	112.1	106.7	117.8	78.3	101.4	96.2
	LOD LOQ (ng/g)	LOD	0.09	0.023	0.19	0.26	0.18	0.088	0.045	0.15	0.34	0.19	0.28	-	2.69
		LOQ	0.27	0.072	0.56	0.79	0.56	0.27	0.14	0.45	1.033	0.58	0.85	4.68	8.89
Non-fat solid	Intra-day precision (%)	2	4.4	3.0	3.8	3.7	2.0	4.0	4.8	5.0	3.2	3.9	4.6	-	2.5
		10	3.2	3.7	4.4	4.5	2.0	2.6	4.2	10.0	4.8	2.6	4.4	4.5	2.4
		20	1.9	2.2	2.3	4.1	2.7	4.8	4.8	4.8	2.8	1.6	4.9	2.0	2.4
	Accuracy (%)	2	95.9	107.7	113.5	108.5	114.9	105.3	108.1	98.2	101.2	108.3	83.3	-	103.9
		10	95.4	107.7	80.5	81.5	98.1	100.7	99.6	71.8	84.7	103.1	94.7	119.1	97.6
		20	97.2	98.1	102.0	97.8	98.6	96.9	83.9	99.1	99.8	108.4	90.1	106.4	95.2
	LOD LOQ (ng/g)	LOD	0.28	0.21	0.29	0.26	0.15	0.28	0.34	0.28	0.21	0.28	0.5	2.32	3.07
		LOD	0.85	0.65	0.87	0.80	0.46	0.84	1.04	0.86	0.65	0.85	1.5	7.03	10.13
Fat solid	Intra-day precision (%)	2.5	10.3	8.2	9.6	4.0	8.4	19.9	19.7	5.9	14.2	12.4	11.5	-	2.2
		12.5	7.2	10.3	6.3	5.3	14.8	5.2	11.6	3.2	2.2	4.1	12.8	13.4	2.0
		25	6.3	6.5	6.0	4.4	6.5	8.1	5.0	7.2	9.7	13.0	9.6	5.5	2.1
	Accuracy (%)	2.5	94.8	107.5	89.8	90.8	115.0	103.9	94.4	93.1	108.5	105.8	113.0	-	85.8
		12.5	98.4	102.0	85.4	90.2	100.3	116.5	95.1	102.6	109.7	99.5	99.5	106.3	100.6
		25	97.4	93.0	77.9	75.9	90.8	85.5	97.0	89.6	98.3	97.0	84.5	75.1	102.9
	LOD LOQ (ng/g)	LOQ	0.64	0.68	0.57	0.23	0.64	1.36	1.23	0.39	1.01	0.87	0.86	2.59	3.00
		LOQ	1.95	2.05	1.73	0.71	1.94	4.13	3.72	1.17	3.08	2.63	2.6	7.86	9.91