Table 3. Accuracy of validated HPLC method for biogenic amines in food matrices.
Matrix | Concentration (mg/kg) | Accuracy (%), n = 5 | AOAC (%)2) | |||||||
---|---|---|---|---|---|---|---|---|---|---|
| ||||||||||
Trp1) | β-phe | Put | Cad | His | Tyr | Spd | Spm | |||
| ||||||||||
Apple juice (Non fatty liquid group) | 10 | 89.85 | 94.62 | 101.90 | 92.42 | 100.90 | 94.54 | 96.83 | 102.89 | 80-115 |
100 | 95.84 | 87.87 | 94.20 | 98.35 | 91.13 | 98.67 | 100.55 | 101.81 | 85-110 | |
500 | 93.92 | 94.83 | 99.93 | 98.31 | 96.85 | 94.89 | 92.54 | 98.31 | 85-110 | |
Juk (Non fatty solid group) | 10 | 108.27 | 102.36 | 94.67 | 109.58 | 101.89 | 102.34 | 109.48 | 104.44 | 80-115 |
100 | 101.05 | 98.39 | 109.97 | 102.11 | 104.77 | 101.92 | 105.95 | 102.44 | 85-110 | |
500 | 94.21 | 96.35 | 100.81 | 101.81 | 98.21 | 95.92 | 99.11 | 95.09 | 85-110 | |
Corn oil (Fatty liquid group) | 10 | 94.05 | 85.97 | 90.49 | 86.57 | 93.88 | 84.85 | 86.60 | 92.79 | 80-115 |
100 | 90.34 | 90.83 | 95.87 | 94.10 | 97.54 | 89.12 | 97.63 | 87.90 | 85-110 | |
500 | 93.05 | 94.83 | 99.13 | 94.41 | 96.14 | 100.23 | 91.83 | 100.97 | 85-110 | |
Peanut butter (Fatty solid group) | 10 | 93.70 | 98.27 | 99.71 | 99.66 | 97.55 | 89.87 | 93.85 | 90.29 | 80-115 |
100 | 96.32 | 91.83 | 96.24 | 102.24 | 100.60 | 97.86 | 103.71 | 91.83 | 85-110 | |
500 | 101.75 | 93.32 | 97.60 | 103.70 | 101.77 | 91.19 | 102.06 | 103.34 | 85-110 |
1)Trp, tryptamine; Phe, β-phenylethylamine; Put, putrescine; Cad, cadaverine; His, histamine; Tyr, tyramine; Spd, spermidine; Spm, spermine.
2)AOAC criteria.