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. 2015 Oct 19;5:15352. doi: 10.1038/srep15352

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Copyright © 2015, Macmillan Publishers Limited

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HUVECs (a,b), A7r5 cells (c,d), and AoSMCs (e,f) were treated with PDGF-BB (a,c,e) or IL-8 (b,d,f) at the indicated concentrations and times. The cell conditioned media and lysates were analyzed by western blotting using anti-HSP60 antibody. Equal protein loading was confirmed with antibody against GAPDH. The results were quantified and plotted in the bar graphs and are expressed as the means ± SD (n = 3). *p < 0.05 compared with the control group. In (a-f), the full length blots were presented in Supplementary Figure S2 (a-f).