Skip to main content
. 2015 Oct 19;5:15213. doi: 10.1038/srep15213

Figure 5. Many endosomes make contacts with the ER.

Figure 5

(a) Electron micrographs of primary cortical neurons treated with a vehicle or 10-NCP (5 μM, 4 h). ER, the endoplasmic reticulum; E, endosome. Note that in an autophagy-stimulated neuron, a large endosome is surrounded by the ER. Bar, 100 nm. (b) In neurons stimulated with 10-NCP, SK1-RFP-positive structures make contacts with EYFP-WIPI1 structures. Neurons transfected with SK1-RFP and WIPI1-YFP were treated with 5 μM 10-NCP (4 h), fixed, and analyzed with confocal microscopy. Bar, 2 μm. Bar in the zoomed image, 0.5 μm. (c) Two S1P-metabolizing enzymes, S1PP and S1PL, are located on the ER. Neurons transfected with S1PP-mApple and Green-ER or with S1PL-mApple and Green-ER were fixed and analyzed by confocal microscopy. Bar, 5 μm. (d) S1PP and S1PL are located on the ER in 10-NCP-treated neurons. Neurons transfected with S1PP-mApple and Green-ER or with S1PL-mApple and Green-ER were treated with 10-NCP (5 μM, 2 h), fixed and analyzed by confocal microscopy. Bar, 5 μm.