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Reconstruction of PDZ domain shuffling in the ZO subfamily. Insertion of the two N-terminal PDZ domains in the common ancestor of ZO2 and ZO3, followed by the insertion of the same two domains, plus the ancestral domain, from ZO2 to ZO1. The ancestral architectures and domain insertions superimposed on the gene tree were reconstructed manually using events and associations of the ancestral nodes in the reconciled domain, gene, and species trees.
