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. Author manuscript; available in PMC: 2015 Oct 19.
Published in final edited form as: Ann N Y Acad Sci. 2010 Aug;1203:120–125. doi: 10.1111/j.1749-6632.2010.05603.x

Figure 1.

Figure 1

Differential effects of UVB on NADPH binding (HP I) and NADPH-independent (HP II) E-coli catalases. Recombinant catalases [(catalase (2.2 μM)] were incubated with 50 μM 2′,7′-dichlorofluorescein diacetate (Molecular Probes, Eugene, OR) and the reaction mixtures were irradiated in uncovered 96-well tissue culture plates (Costar, Corning, NY) with UVB light emitted from two Westinghouse FS20 light tubes. The UVB lights were calibrated with an IL 442A Phototherapy Radiometer (International Light, Newburyport, MA). Fluorescence was quantified using an HTS 7000 plus bio-assay reader (PerkinElmer Life Sciences, Beaconsfield Buckinghamshire, UK) with 495 nm excitation and 520 nm emission filters. E. coli hydroperoxidases were generously provided by Peter Loewen, U. Manotoba).