Table 1.
Amplification primers used to obtain the mitochondrial genome of Cyclospora cayetanensis and confirm its linear nature.
| FRAGMENT | PRIMER ID | NUCLEOTIDE SEQUENCE (5’-3’) | SIZE in bp (position within mt genomea) |
REFERENCE |
|---|---|---|---|---|
| Fragment 1 | Cocci_MT-WG-F Cyclo_COI_473R |
TACACCTAGCCAACACGAT ATACCCGCAAGAGCTAAACC |
1807 (23 - 1829) |
Ogedengbe et al., 2014b Ogedengbe et al., 2013 |
| Fragment 2 | qPCR400-F COI_1202R |
GDTCAGGTRTTGGTTGGAC CCAAKRAYHGCACCAAGAGATA |
803 (1723 - 2525) |
Ogedengbe et al., 2013
Ogedengbe et al., 2013 |
| Fragment 3 | Cyclo_COI-1085F Cyclo_CO3_113R |
CTCCGCTCTAGATGTTGCTT TCACCATTCTTGCTCACTGT |
2030 (2442 - 4471) |
This Study This Study |
| Fragment 4 | WG-MT_4140F Eim_CO3_799R |
AGAAAACCTAAAATCATCATGT AAGTGAGTTCGCATGTTTAC |
961 (4191-5151) |
Ogedengbe et al., 2014b This Study |
| Fragment 5 | Cyclo_CO3_219F Cocci_MT-WG-R |
AGCTTCTTCTGGGGTGCATAC GCAGCTGTAGATGGATGCTT |
1607 (4578 - 6184) |
This Study Ogedengbe et al., 2014b |
| WG_MT_344R Cocci_MT_WG_F (Positive Control) |
GTAGGAATCTRAATTCCCAACC TACACCTAGCCAACACGAT |
431 (23-453) |
Ogedengbe et al., 2013
Ogedengbe et al., 2014b |
|
|
PCR confirmation of
linear genome |
WG_MT_344R WG_MT_6219F |
GTAGGAATCTRAATTCCCAACC GCATCCATCTACAGCTGCGG |
No Product |
Ogedengbe et al., 2013
Ogedengbe et al., 2013 |
| WG_MT_344R WG_MT_5416F |
GTAGGAATCTRAATTCCCAACC GGTCCAGATAAGCGATCTCATG |
No Product |
Ogedengbe et al., 2013
Ogedengbe et al., 2013 |
a
Positions reported are relative to the complete mitochondrial (mt) genome of C. cayetanensis (GenBank KP658101); complete 5’-end was generated using terminal deoxynucleotidyl transferase tailing followed by nested PCR and sequencing (see Section 2.3) but 3’-terminus has not been similarly completed.