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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Biomol Screen. 2014 Dec 22;20(5):655–662. doi: 10.1177/1087057114564057

Figure 5. The two most potent inhibitors of PIP5K1C have the same core structure and UNC3230 exhibits ATP-competitive mode of inhibition.

Figure 5

(A) Compound 1 (UNC3230) and (B) Compound 2 (UNC2828) have the same thiazole carboxamide core structure and IC50 values of 120 and 130 nM, respectively. Mean ± SD. n=3 reactions per data point. (C) Progression curves for ATP competition studies with PIP5K1C and various concentrations of UNC3230. (D) Plot of Kmapp/Vmaxapp to determine inhibitory constant, Ki. (E) Plot of Kmapp and (F) Vmaxapp against increasing concentrations of UNC3230 to determine mode of inhibition.