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. 2015 Sep 14;4(10):1290–1297. doi: 10.1242/bio.011874

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Phosphorylation of Ser-55 of vimentin is required for Rac1 effect on mitochondria motility. Cells MFT-16 were transfected either with plasmids derivative of pIRES2-EGFP with inserted cDNAs encoding Vim(WT) (control), Vim(S55A), Vim(S54A), or Vim(S38A) or plasmids derivative of pIRES2-EGFP-Rac1(G12V) with inserted cDNAs encoding Vim(WT), Vim(S55A), Vim(S54A), or Vim(S38A). 18 h after transfection cells were stained with MitoTracker Red and movements of mitochondria were analyzed. Values are mean percentage of movements exceeding 0.2 μm/s±s.e.m.; n=number of cells (approximately 500 movements in each cell). *Significantly different from control (P<0.01); **no significant difference from control (P>0.80).