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. 2015 Oct 1;8(10):1311–1321. doi: 10.1242/dmm.019398

Fig. 4.

Fig. 4.

Effects of HG and albumin on DAMP release and gene expression in mPTCs. (A) Immunoblot analyses of HSP70 and HMGB1 from the cell lysate and culture medium of mPTCs treated with LG, HG and different concentrations of albumin for 24 h. (B) Immunoblot analyses of HSP70 and HMGB1 from the culture medium of mPTCs treated with various sources of albumin (2 mg/ml) as described for Fig. 3D. Expression of Ccl2 and Tnfα in WT mPTCs pretreated with pifithrin-μ (C), VER-155008 (D) and KNK437 (E) for 30 min before albumin stimulation. n=3-4 in each group. Expression of Tlr2 and Tlr4 (F) and Cubn and Lrp2 (G) in WT mPTCs treated with LG, HG and albumin (0.2 mg/ml) for 24 h. *P<0.05, **P<0.01 and ***P<0.001 by one-way ANOVA followed by Dunnett's test.