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. 2015 Oct 1;8(10):1265–1278. doi: 10.1242/dmm.019489

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Altered APP-related secretase expression occurs in hAPPsw^+/+ RG-differentiated astrocytes but does not lead to an increase in Aβ production. (A) Western blot using the anti-APP(A4) revealed a significant decrease in sAPP following differentiation of WT RGs but no change in sAPP in the hAPPsw RGs. Expression differences are shown as adjusted density following normalization to β-Actin. A typical western blot is shown. Error bars denote s.d. (B) Western blot using the anti-APP(A4) revealed the presence of immature APP in both WT and hAPPsw RGs. A typical western blot is shown. Error bars denote s.d. (C) ADAM10 α-secretase expression is decreased in both undifferentiated hAPPsw^+/+ RGs and in astrocytes. A typical western blot is shown. Student's t-test was performed on biological replicates and significance was found when P≤0.05. Error bars denote s.d. (D) BACE1 β-secretase expression is decreased in hAPPsw^+/+ astrocytes compared with WT astrocytes. A typical western blot is shown. Student's t-test was performed on biological replicates and significance was found when P≤0.05. Error bars denote s.d. (E) PEN2, γ-secretase expression is increased in hAPPsw^+/+ differentiated RGs compared with WT RGs. A typical western blot is shown. Student's t-test was performed on biological replicates and significance was found when P≤0.05 (marked by *). Error bars denote s.d. (F) Levels of secreted Aβ40 and Aβ42 peptides increase during differentiation into astrocytes but are not significantly different between hAPPsw and WT cells. Student's t-test was performed on biological replicates and significance was found when P≤0.05 (marked by *). Error bars denote s.d. (G) The Aβ40:Abβ2 of secreted peptides increased in hAPPsw^+/− astrocytes compared with undifferentiated hAPPsw^+/− astrocytes but this was not significantly higher than WT astrocytes. Student's t-test was performed on biological replicates and significance was found when P≤0.05 (marked by *).