Fig. 4.

Loss of fos or knockdown of ets21c partly suppresses dilp8 expression, and simultaneous TF inhibition further improves pupation timing. (A) Suppression of ets21c^LONG and fos in ras^V12scrib¹ tumors improved timing and progression of pupation by 1 day compared with the single knockdowns (P<0.0001; dashed lines repeated from Fig. 3). A mild improvement is also observed upon combined knockdown of ets21c^LONG and ftz-f1 (P<0.005). Some ras^V12scrib¹ftz-f1^RNAiets21c^{LONG RNAi} animals eclosed as adults, whereas ras^V12scrib¹kay³ets21c^{LONG RNAi} individuals all died as pupae. The graph shows the cumulative percentage of pupae forming over time. (B) Elevated expression of dilp8 mRNA in ras^V12scrib¹ mosaic EAD was reduced upon JNK inhibition, loss of fos or ets21c^LONG knockdown, but not in ras^V12scrib¹ftz-f1^RNAi tumors. Data are mean values±s.e.m.; n=3-5; ***P<0.001; **P<0.005; *P<0.01.