Table 1.
Primer sequences and characterization of 31 microsatellite loci isolated from Jasminum sambac.
| Locus | Primer sequences (5′–3′) | Repeat motif | Ta (°C) | Allele size (bp) | A | GenBank accession no. |
| Js004 | F: CCAAATTGTCATTGGGCTCT | (CTT)5 | 48 | 214 | 1 | KR339145 |
| R: GCTAGCTTTGATGGGTTGGA | ||||||
| Js010 | F: TGCGAAGACTCTCAGCAGAA | (AG)6 | 48 | 189 | 1 | KR339151 |
| R: AACAGCTTCACGCTCTCCTC | ||||||
| Js011 | F: AACATCCAAACAGGCCAAAA | (CA)9 | 48 | 206 | 1 | KR339152 |
| R: CAGAAGGAATCCACCCTTCA | ||||||
| Js012 | F: GACGGTCGGTCCTCACTTTA | (GAC)5 | 48 | 228 | 1 | KR339153 |
| R: ACTTGAATGGATCAAACGGC | ||||||
| Js016 | F: CCATTGGCTGGAGAGATGAT | (TAC)6 | 48 | 251 | 1 | KR339157 |
| R: CCCACTGCCAAGTCCTTTTA | ||||||
| Js020 | F: ACATGAATCGAGGAAAACCG | (TTC)5 | 48 | 269 | 1 | KR339161 |
| R: GAATGGCGAAGGAAAATGAA | ||||||
| Js021 | F: GAAGAGGGAACTACCCGGTC | (GT)6 | 48 | 275–285 | 2 | KR339162 |
| R: ATGAGAGCAAAGAGGGGACA | ||||||
| Js029 | F: TTCTCTCACATCGGTGGTTG | (ATGT)5 | 48 | 278 | 1 | KR339170 |
| R: TGACAAGAACCAAACCAATCC | ||||||
| Js030 | F: AACTCCGTGTACTCCGCAAG | (GA)7 | 48 | 245–251 | 2 | KR339171 |
| R: GCGAAAATCAAACTGCCATC | ||||||
| Js033 | F: GAAATCTGATGCTGCAACCA | (AT)7 | 48 | 256–262 | 2 | KR339174 |
| R: AAAGAGTTCATCCATTCGGG | ||||||
| Js035 | F: GACTTTGCGAGGGAAAACAG | (TGG)6 | 48 | 252–254 | 2 | KR339176 |
| R: CCAACCCTTCGACTCCTACA | ||||||
| Js040 | F: GTAGATTCGGCGTTACTCGG | (AAG)6 | 48 | 204–213 | 2 | KR339181 |
| R: CTTTTCTTCATAGCCCGACG | ||||||
| Js041 | F: GGAATTGTGGATGGCTCACT | (CAAA)5 | 48 | 136–142 | 3 | KR339182 |
| R: TGAGAGTTGGATGGGCTTTT | ||||||
| Js042 | F: AGAAATTTTTCCGGCTACGG | (CCG)5 | 48 | 212–218 | 3 | KR339183 |
| R: CCCATGACTAACCCGGTAGA | ||||||
| Js050 | F: TCCAAGAAAATGAACGGGAT | (TAC)5 | 48 | 268 | 1 | KR339191 |
| R: TGAATTGGCCTATCCTTTGG | ||||||
| Js055 | F: TGTTGCTCCTTCACATCAGC | (CTC)6 | 48 | 171–180 | 2 | KR339196 |
| R: GCCCCATCGTAGGGTAAAAT | ||||||
| Js057 | F: CTGATCTCTGCCACGTTCAA | (CCG)5 | 48 | 278 | 1 | KR339198 |
| R: AAAATAACAAAAATCCTCCGCA | ||||||
| Js061 | F: TCTTGGGTTGGCTTCAGAAC | (AGC)5 | 48 | 242 | 1 | KR339202 |
| R: CAGCGAAGTGAGTCTGGTCA | ||||||
| Js062 | F: TGAAACTACCGGGTCTTTGG | (AGA)5 | 48 | 230 | 1 | KR339203 |
| R: ATTAATCGGTCCTGAAGGGG | ||||||
| Js063 | F: CCCATCTCACCCTAACCTCA | (TA)6 | 50 | 188–194 | 2 | KR339204 |
| R: GAAAAATTCTTGGATCTTCTTGC | ||||||
| Js064 | F: TCAACGCCTTAAATTGCTTG | (CT)6 | 48 | 275–281 | 2 | KR339205 |
| R: CCACAAACCTTCGAGGAGAC | ||||||
| Js068 | F: ATAAAGCACAACATCCCGCT | (AG)6 | 48 | 123 | 1 | KR339209 |
| R: TTTCTCACTCCGGCACTTCT | ||||||
| Js073 | F: GCCTCGAAATGTTGGAATGT | (GAA)6 | 48 | 213 | 1 | KR339214 |
| R: CCAAACTACGAAGGGGAAAA | ||||||
| Js075 | F: ACGCAATCTACCCCATTGAG | (GAT)5 | 48 | 266 | 1 | KR339216 |
| R: TCCTTCAGCAACATTGCATC | ||||||
| Js076 | F: TGTAACGCGAAACGGAATTT | (TGG)6 | 48 | 163–172 | 2 | KR339217 |
| R: ACCAACCACGGTGTTTCTTC | ||||||
| Js077 | F: TTTGCCATCAATGTCTCCCT | (GT)9 | 48 | 143 | 1 | KR339218 |
| R: CCTGTTCCTGTTTCCATCAGA | ||||||
| Js079 | F: CAAGAAAATTGACCCCATCG | (TA)7 | 48 | 157–163 | 2 | KR339220 |
| R: GACTTGGTCGCCATTGTTTT | ||||||
| Js084 | F: TCATAACCCCTCGCTTTTTG | (CCA)5 | 48 | 212 | 1 | KR339225 |
| R: AAGCTTGGGGGAGGAAGTTA | ||||||
| Js085 | F: CCATGGACAAACATTGTGGA | (AAC)5(AGC)5 | 48 | 204–207 | 2 | KR339226 |
| R: TTTCAAAGAGCGGAACCAGT | ||||||
| Js086 | F: TCCACCTCTCGGGTTATTTG | (GAA)6 | 48 | 150 | 1 | KR339227 |
| R: AAAGTCTGCAAAAGGGAGCA | ||||||
| Js100 | F: AAGCATCGAGAATCGAGCATA | (AG)6 | 48 | 230 | 1 | KR339241 |
Note: A = number of alleles; Ta = PCR annealing temperature.