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. Author manuscript; available in PMC: 2015 Oct 19.
Published in final edited form as: Hepatology. 2012 Jun;55(6):1863–1875. doi: 10.1002/hep.25572

Fig. 4. HMGB1 is necessary for hypoxia-induced activation of caspase-1 and induces activation of caspase-1 in normoxia.

Fig. 4

(A) Western blot analysis for caspase-1 from Hepa1-6 cells treated with 10 mM ethyl pyruvate (EP) or 1 μg/ml anti-HMGB1 neutralizing antibody (a-HMGB1) in hypoxia. The blot shown is representative of three experiments with similar results. (B) Caspase-1 activity was determined by colorimetric assay after various treatments. *P < 0.05 versus Hepa1-6 cells that were subjected to hypoxia. (C) Western blot analysis of caspase-1 in Hepa 1-6 cells treated with various dose rhHMGB1 for 24h. (D) Western blot analysis of caspase-1 in Hepa1-6 cells treated with 1 μg/ml rhHMGB1 for various times. (E) Ectopic overexpression of HMGB1-GFP fusion protein in Hepa1-6 stable clones. GFP and HMGB1-GFP fusion expression were confirmed via western blot analysis with GFP antibody. (F) Cleaved caspase-1 was significantly increased in HMGB1 stable transfectants as compared with vector controls via western blot analysis.