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. Author manuscript; available in PMC: 2015 Oct 19.
Published in final edited form as: Hepatology. 2012 Jun;55(6):1863–1875. doi: 10.1002/hep.25572

Fig. 5. Signaling pathways downstream of HMGB1 are involved in hypoxia-induced activation of caspase-1.

Fig. 5

(A) Expression of TLR4 from Hepa1-6 cells subjected to hypoxia for various time points. (B) Western blot analysis for caspase-1 from hypoxic Hepa1-6 cells transfected with scrambled siRNA or TLR4 siRNA or treated with 5 μg/ml anti-TLR4 neutralizing antibody (a-TLR4). (C) Expression of RAGE from Hepa1-6 cells subjected to hypoxia for various time points. (D) Western blot analysis for caspase-1 from hypoxic Hepa1-6 cells transfected with scrambled siRNA or RAGE siRNA or treated with 5 μg/ml sRAGE. (E) Western blot analysis for caspase-1 from Hepa1-6 cells treated with a-TLR4 or/and sRAGE. The blot shown is representative of three experiments with similar results.