Fig. 4.
Kinetic assessment of Lipofermata inhibition of fatty acid uptake in HepG2 cells. The fatty acid analogue C1-BODIPY-C12 was used to evaluate the mechanism of Lipofermata inhibition of fatty acid transport. The inserted legend indicates the concentrations in µM of Lipofermata used to generate the lines. Data points are the mean and the error bars indicate the standard deviation. As shown, the data were best fit to a noncompetitive inhibition model. Analysis was performed using SigmaPlot 12 enzyme kinetics module.