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IgG subclasses IgG1 (A) and IgG2a (B) in sera of mice that received IRV by MN or IM administration. Pooled sera on day 28 were tested by EIA for IgG1 and IgG2a as described in the text. Data showing the ratio of IgG2a and IgG1 are shown in panel C. The character “*” indicates IRV reconstituted from coated MN.
