. Author manuscript; available in PMC: 2016 Oct 20.

Published in final edited form as: Eur J Med Chem. 2015 Sep 5;103:600–614. doi: 10.1016/j.ejmech.2015.08.021

Table 2.

Parameters from inhibition kinetics of E. coli DHFR.

Inhibitors	Substrate	Inhibition	K_i/αK_i (nM)^*
NSC120927	H₂F	Competitive	42.50 ± 5.34
NSC120927	NADPH	Uncompetitive	946.4 ± 62.3
NSC132279	H₂F	Competitive	100.9 ± 12.7
NSC132279	NADPH	Uncompetitive	2019 ± 155
NSC132277	H₂F	Competitive	394.3 ± 58.4
NSC132277	NADPH	Uncompetitive	7432 ± 375
NSC133071	H₂F	Competitive	430 ± 53.8
NSC133071	NADPH	Uncompetitive	12300 ± 793
NSC113909	H₂F	Competitive	3816.0 ± 434.0
NSC113909	NADPH	Uncompetitive	62240 ± 3557

The K_i is reported for competitive inhibition while αK_i is reported for uncompetitive inhibition;