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. Author manuscript; available in PMC: 2016 Sep 1.
Published in final edited form as: Cell Mol Bioeng. 2015 May 28;8(3):333–348. doi: 10.1007/s12195-015-0397-4

FIGURE 4.

FIGURE 4

Expression of calponin and smoothelin has greater dependence on soluble factors than ECM. Representative Western blots for calponin (a) and smoothelin (c) after each step of priming and stimulation with growth or differentiation medium. SS: Serum-starve, TGFβ: TGFβ1 treatment step, DM: switch to differentiation medium, GM: switch to growth medium. Internal control bands for normalization are cyclophilin B (Cy. B). Immunofluorescent staining of calponin (b) and smoothelin (d) (green) with actin (red) and DAPI (blue). Scale bar is 50 μm. (e) Reverse transcriptase PCR to confirm expression of SMTN-B mRNA.