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KM, Vmax, kcat and kcat/KM values for Hb, Hb-PAA-450k(1:0.8:1.5) and Hb-PAA-450k(1:0.3:1.5) samples. Vmax values of Hb-PAA conjugates are comparable to Hb and Km values decreased considerably for Hb-PAA conjugates in comparison to Hb.
| KM | Vmax | kcat | kcat/KM | |
|---|---|---|---|---|
| Hb | 0.76 mM | 0.345·µM·s−1 | 0.345·s−1 | 4.54 × 102 M−1·s−1 |
| Hb-PAA-450k(1:0.8:1.5) | 0.10 mM | 0.332·µM·s−1 | 0.332·s−1 | 3.32 × 103 M−1·s−1 |
| Hb-PAA-450k(1:0.3:1.5) | 0.15 mM | 0.383·µM·s−1 | 0.383·s−1 | 2.55 × 103 M−1·s−1 |
