Table 2.
T-cell responses the day before the genital challenge1
| Antigen | Adjuvant | T-cell proliferation responses to: | In vitro cytokine production | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| EB2 | Con A3 | Medium | IFN-γ (pg/ml) | IL-4 (pg/ml) | |||||||
| CPM x103 | SI4 | CPM x103 | SI4 | CPM x103 | SI4 | EB2 | Con A3 | EB2 | Con A3 | ||
| MOMP | CpG+Montanide | 1·3 ± 0·75 | 9·45,6 | 32·3 ± 9·2 | 223 | 0·2 ± 0·1 | 1 | 6682 ± 4025,6 | 77 892 ± 6850 | <0·8 | <0·8 |
| Ovalbumin | CpG+Montanide | 0·8 ± 0·7 | 1·9 | 29·0 ± 7·7 | 80 | 0·4 ± 0·2 | 1 | 4792 ± 612 | 80 668 ± 4650 | <0·8 | <0·8 |
| MOMP | CpG+Alum | 0·3 ± 0·1 | 2·0 | 33·9 ± 14·3 | 211 | 0·2 ± 0·1 | 1 | 2308 ± 724 | 76 805 ± 2987 | <0·8 | <0·8 |
| Ovalbumin | CpG+Alum | 0·5 ± 0·2 | 2·4 | 20·9 ± 5·7 | 99 | 0·2 ± 0·1 | 1 | 2272 ± 498 | 80 341 ± 1687 | <0·8 | <0·8 |
| Cm EB | 6·9 ± 2·85 | 80·05 | 36·6 ± 10·1 | 391 | 0·1 ± 0·02 | 1 | 72 149 ± 13 9105 | 71 820 ± 3964 | <0·8 | <0·8 | |
| MEM | 0·3 ± 0·1 | 2·4 | 43·3 ± 15·3 | 309 | 0·1 ± 0·1 | 1 | 1883 ± 791 | 70 052 ± 3863 | <0·8 | <0·8 | |
ConA, concanavalin A; EB, elementary body; IFN-γ, interferon-γ; IL-4, interleukin-4; MEM, minimal essential medium; MOMP, major outer membrane protein.
Results are means for triplicate cultures (±1 SD). Data correspond to one of the experiments representative of duplicate separate experiments.
UV-inactivated Chlamydia muridarum EB were added at a 10 : 1 ratio to the antigen-presenting cells.
Concanavalin A was added at a concentration of 5 µg/ml.
SI = stimulation index (CPM of EB stimulated/CPM of medium stimulated).
P < 0·05 by the Student's t-test, compared with the corresponding MEM-immunized control group.
P < 0·05 by the Student's t-test, compared with the corresponding ovalbumin-immunized control group.