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. 2015 Oct 5;146(3):470–485. doi: 10.1111/imm.12524

Figure 6.

Figure 6

Interferon-γ (IFN-γ) enhances Staphylococcus aureus binding and increases Toll-like receptor (TLR) and scavenger receptors on human mast cells (huMCs). Human MCs were pre-treated with IFN-γ (20 ng/ml) for 48 hr before addition of FITC-labelled S. aureus (20 : 1) (a) or antibodies to surface receptors (b) and analysed by flow cytometry. Results are representative flow cytometric traces or means ± SE performed in duplicate, n = 3 huMC donors. Differences between individual groups were tested for statistical significance by paired Student's t-test (*P ≤ 0·05, **P ≤ 0·01 and ***P ≤ 0·001 for comparison between control and IFN-γ-stimulated cells).