Table 1.
Experimental Design a | Human | Mouse | Rat | ||||||
---|---|---|---|---|---|---|---|---|---|
Protein | Peptide | FDR | Protein | Peptide | FDR | Protein | Peptide | FDR | |
Experiment 1 | 4,014 | 9,455 | 1.2% | 4,048 | 9,372 | 1.7% | 4,079 | 9,120 | 1.5% |
Experiment 2 | 4,293 | 8,742 | 0.5% | 4,226 | 8,608 | 1.4% | 4,381 | 8,530 | 1.3% |
The raw data from Experiments 1 and 2 were independently searched against human, mouse and rat sequences in the UniRef 100 database, using the search results combined from Mascot (v2.3) and Sequest search engines through the Proteome Discoverer software (v1.3). The resulting files were imported into Scaffold for both qualitative and quantitative analyses. The number of proteins and peptides identified from each database search are listed in this table, following stringent data filtering described in Materials and Methods.