Figure 2.

PF downregulated STAT3 and p-STAT3 protein expression in U87 and U251 cells.

Notes: (A–D) The concentration course study for the expression of total and phosphorylated STAT3 by immunoblot assay. (E–H) Cells were treated with 20 mM PF for indicated time points (0, 6, 12, and 24 hours). Cell lysates were subjected to Western blot with the indicated antibodies. (B, D, F, H) Quantification of protein levels normalized to β-actin. The data are expressed as mean ± SD of three independent experiments, and significant differences from the control are indicated by ^*P<0.05.

Abbreviations: PF, paeoniflorin; STAT3, signal transducer and activator of transcription 3.