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. 2015 Oct 13;9:5611–5622. doi: 10.2147/DDDT.S93912

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© 2015 Nie et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Resistance to PF-induced apoptosis in STAT3-overexpressing glioma cells.

Notes: Group definition: empty vector transfected cells with vehicle alone (a) or with PF treatment (b); STAT3-expressing plasmid transfected cells with vehicle exposure (c) or with the PF exposure (d). (A) U87 cells were transfected with STAT3-expressing plasmid or empty vector as described and confirmed by Western blot analysis. (B) Quantification of protein levels normalized to β-actin. (C) Cell extracts from each group were immunoblotted for STAT3 and p-STAT3. (D) Quantification of protein levels normalized to β-actin. (E) Cell viability was determined by MTT assay. (F–G) Cells were stained with Annexin V-FITC/PI and analyzed by flow cytometry. ^*P<0.05 compared with group a. ^#P<0.05 compared with group b. The data are representative of three separate experiments and presented as the mean ± SD; error bars indicate SD.

Abbreviations: PF, paeoniflorin; STAT3, signal transducer and activator of transcription 3; MTT, thiazolyl blue tetrazolium bromide.

Resistance to PF-induced apoptosis in STAT3-overexpressing glioma cells.

Notes: Group definition: empty vector transfected cells with vehicle alone (a) or with PF treatment (b); STAT3-expressing plasmid transfected cells with vehicle exposure (c) or with the PF exposure (d). (A) U87 cells were transfected with STAT3-expressing plasmid or empty vector as described and confirmed by Western blot analysis. (B) Quantification of protein levels normalized to β-actin. (C) Cell extracts from each group were immunoblotted for STAT3 and p-STAT3. (D) Quantification of protein levels normalized to β-actin. (E) Cell viability was determined by MTT assay. (F–G) Cells were stained with Annexin V-FITC/PI and analyzed by flow cytometry. ^*P<0.05 compared with group a. ^#P<0.05 compared with group b. The data are representative of three separate experiments and presented as the mean ± SD; error bars indicate SD.

Abbreviations: PF, paeoniflorin; STAT3, signal transducer and activator of transcription 3; MTT, thiazolyl blue tetrazolium bromide.