Figure 4.

Time course of macrophage internalized in 110 nm PVP-suspended AgNPs.

Notes: Macrophages were exposed to AgNPs, AgNPs with a complex PC, or AgNPs with a BSA PC at a concentration of 25 µg/mL for 30 minutes. Following exposure, AgNPs were removed, and cells were washed and placed in media with serum. Spectra of internalized 110 nm AgNPs were assessed at 30 minutes, 2 hours, 6 hours, and 24 hours postexposure.

Abbreviations: PVP, polyvinylpyrrolidone; NPs, nanoparticles; PC, protein corona; BSA, bovine serum albumin; h, hours; min, minutes.