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. 2015 Oct 13;10:6509–6521. doi: 10.2147/IJN.S92570

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© 2015 Shannahan et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Measurement of AgNP uptake by assessment of changes in SSC via flow cytometry and ICP-MS.

Notes: Macrophages were exposed to AgNPs, AgNPs with a complex PC, or AgNPs with a BSA PC at a concentration of 25 µg/mL for 2 hours, and uptake was determined by (A) assessment of changes in SSC shift and (B) ICP-MS analysis of silver content. SSC values of cells exposed to AgNPs were normalized to serum-free media control cells to produce a fold change. Values are expressed as mean ± SEM (n=4–7 per group). *Significant difference from unexposed control cells (P<0.05). ^#Significant difference compared to AgNP without a PC (P<0.05).

Abbreviations: NP, nanoparticle; SSC, side scatter; ICP-MS, inductively coupled plasma-mass spectrometry; PC, protein corona; BSA, bovine serum albumin; SEM, standard error of mean; PVP, polyvinylpyrrolidone.