Figure 1.

Generation of PLD1^−/− and PLD2^−/− mice. (A). Targeting constructs. The neo gene was removed by the FLP recombinase. The Cre-loxP system was used to delete exon 11 of PLD1 or exons 11 and 12 of PLD2. These exons were floxed by two LoxP sites. (B). Absence of PLD1 and PLD2 protein in PLD-deficient mice. BMMCs derived from the bone marrow cells of dKO (PLD1^−/−PLD2^−/−), PLD1^−/−, PLD2^−/−, and WT mice were analyzed by Western blotting after anti-PLD1 and anti-PLD2 immunoprecipitation.