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The effect of PLD deficiency on the PI3K pathway. dKO, PLD1−/−, PLD2−/−, and WT BMMCs were sensitized with anti-DNP IgE and activated with DNP-HSA before lysis. Whole cell lysates were blotted with antibodies against the phosphorylated and non-phosphorylated forms of PDK1, p70S6K, and AKT. Data shown are representative of three experiments. The numbers shown were relative intensities for the phosphorylated form of proteins normalized by non-phosphorylated form.
