TABLE 1 .
Assessment of VirCapSeq-VERT efficiency by using quantitated viral nucleic acids to spike lung and blood host nucleic acid
| Pool | Host background | Virusb | Loada | Library preparation |
|---|---|---|---|---|
| 1a | 200 ng lung NA | FLUAV (Orthomyxoviridae); segmented negative-strand RNA, 13 kb/8 segments |
2 × 104 | Conventional/HTS |
| MERS-CoV (Nidovirales, Coronaviridae); nonsegmented positive-strand RNA, 30 kb |
2 × 104 | |||
| EV-D68 (Picornavirales, Picornaviridae); nonsegmented positive-strand RNA, 7 kb |
3 × 105 | |||
| 1b | Same as pool 1a | Same as pool 1a | Same as pool 1a | VirCapSeq-VERT |
| 2a | 200 ng blood NA | DENV-3 (Flaviviridae); nonsegmented positive-strand RNA, 11 kb |
5 × 105 | Conventional/HTS |
| WNV (Flaviviridae); nonsegmented positive-strand RNA, 11 kb |
9 × 103 | |||
| EBOV (Mononegavirales, Filoviridae); nonsegmented negative-strand RNA, 19 kb |
2 × 103 | |||
| CVV (Bunyaviridae); segmented negative-strand RNA, 12 kb/3 segments |
8 × 103 | |||
| HHV-1 (Herpesvirales, Herpesviridae); nonsegmented double-strand DNA, 152 kb |
2 × 105 | |||
| 2b | Same as pool 2a | Same as pool 2a | Same as pool 2a | VirCapSeq-VERT |
a
Determined by qPCR of double-stranded cDNA/DNA used for sequence library construction.
b
FLUAV, influenza A virus H3N2; MERS-CoV, Middle East respiratory syndrome coronavirus; EV-D68, enterovirus D68; DENV-3, dengue virus 3; WNV, West Nile virus; EBOV, Ebola virus; CVV, Cache Valley virus; HHV-1, herpes simplex virus 1.