TABLE 2 .
VirCapSeq-VERT provides greater genome coverage and sequencing depth than HTS
| Libraryb | Virus | Load (copies)a | Genome length (nt) | No. of mapped positions | % sequence mapped | Coverage |
No. of unmapped regions | Unmapped region length (nt) | ||
|---|---|---|---|---|---|---|---|---|---|---|
| Min | Max | Avg | ||||||||
| Pool 1a (lung, HTS) | EV-D68 | 105 | 7,341 | 7,268 | 99.01 | 0 | 2,384 | 932 | 4 | 73 |
| MERS-CoV | 104 | 30,113 | 1,824 | 6.06 | 0 | 2 | 0.1 | 19 | 28,289 | |
| FLUAV -1 | 104 | 2,316 | 2,005 | 86.57 | 0 | 9 | 2.5 | 5 | 311 | |
| FLUAV -2 | 2,304 | 2,248 | 97.57 | 0 | 19 | 6.4 | 2 | 56 | ||
| FLUAV -3 | 2,208 | 1,998 | 90.49 | 0 | 29 | 3.8 | 4 | 210 | ||
| FLUAV -4 | 1,737 | 1,642 | 94.53 | 0 | 32 | 8.0 | 2 | 95 | ||
| FLUAV -5 | 1,540 | 1,494 | 97.01 | 0 | 14 | 4.1 | 3 | 46 | ||
| FLUAV -6 | 1,442 | 1,334 | 92.51 | 0 | 11 | 4.2 | 3 | 108 | ||
| FLUAV -7 | 1,002 | 948 | 94.61 | 0 | 11 | 3.7 | 2 | 54 | ||
| FLUAV -8 | 865 | 801 | 92.60 | 0 | 11 | 3.8 | 3 | 65 | ||
| Pool 1b (lung, VirCapSeq-VERT) | EV-D68 | 105 | 7,341 | 7,341 | 100.00 | 3 | 8,080 | 7,005 | 0 | 0 |
| MERS-CoV | 104 | 30,113 | 29,020 | 96.37 | 0 | 121 | 13 | 23 | 1,093 | |
| FLUAV -1 | 104 | 2,316 | 2,316 | 100.00 | 590 | 8,061 | 5,230 | 0 | 0 | |
| FLUAV -2 | 2,304 | 2,304 | 100.00 | 569 | 8,048 | 7,608 | 0 | 0 | ||
| FLUAV -3 | 2,208 | 2,208 | 100.00 | 818 | 8,040 | 4,847 | 0 | 0 | ||
| FLUAV -4 | 1,737 | 1,737 | 100.00 | 323 | 8,038 | 7,449 | 0 | 0 | ||
| FLUAV -5 | 1,540 | 1,540 | 100.00 | 909 | 8,003 | 7,091 | 0 | 0 | ||
| FLUAV -6 | 1,442 | 1,442 | 100.00 | 348 | 7,999 | 6,975 | 0 | 0 | ||
| FLUAV -7 | 1,002 | 1,002 | 100.00 | 60 | 8,056 | 6,216 | 0 | 0 | ||
| FLUAV -8 | 865 | 865 | 100.00 | 448 | 8,006 | 5,761 | 0 | 0 | ||
| Pool 2a (blood, HTS) | HHV-1 | 105 | 152,151 | 151,970 | 99.88 | 0 | 418 | 142 | 4 | 183 |
| DENV-3 | 105 | 10,707 | 10,687 | 99.81 | 0 | 1,242 | 622 | 1 | 20 | |
| WNV | 104 | 10,945 | 500 | 4.57 | 0 | 1 | 0.1 | 6 | 10,445 | |
| EBOV | 103 | 18,959 | 4,716 | 24.87 | 0 | 2 | 0.3 | 43 | 14,243 | |
| CVV-S | 104 | 905 | 818 | 90.39 | 0 | 7 | 3.1 | 3 | 87 | |
| CVV-M | 4,305 | 2,633 | 61.16 | 0 | 5 | 1.1 | 15 | 1,672 | ||
| CVV-L | 6,840 | 2,309 | 33.79 | 0 | 5 | 0.5 | 17 | 4,531 | ||
| Pool 2b (blood, VirCapSeq-VERT) | HHV-1 | 105 | 152,151 | 152,133 | 99.99 | 0 | 8,001 | 5,373 | 1 | 18 |
| DENV-3 | 105 | 10,707 | 10,688 | 99.82 | 0 | 8,068 | 7,774 | 1 | 19 | |
| WNV | 104 | 10,945 | 10,428 | 95.28 | 0 | 214 | 66 | 1 | 517 | |
| EBOV | 103 | 18,959 | 16,413 | 86.57 | 0 | 394 | 56 | 11 | 2,546 | |
| CVV-S | 104 | 905 | 904 | 99.89 | 0 | 7,319 | 2,302 | 1 | 1 | |
| CVV-M | 4,305 | 4,305 | 100.00 | 2 | 1,551 | 401 | 0 | 0 | ||
| CVV-L | 6,840 | 6,840 | 100.00 | 1 | 858 | 88 | 0 | 0 | ||
a
Determined by qPCR of double-stranded-cDNA/DNA used for sequence library construction.
b
See Table 1 for pool composition.