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. 2015 Sep 22;6(5):e01491-15. doi: 10.1128/mBio.01491-15

TABLE 5 .

Genome mapping and coverage in VirCapSeq-VERT multiplex assays

Virus 7-plex mixa
 21-plex mix
Load (copies) %genome mapped Avg coverage Load (copies) % genome mapped Avg coverage Load (copies) % genome mapped Avg coverage Load (copies) % genome mapped Avg coverage
HHV-1 104 100 4,258 104 99.6 583 106 99.9 5,438 102 84.5 10
MERS-CoV 104 27.9 1.1 104 20.1 0.34 106 98.7 23 102 0.3 0
WNV 104 98.8 4,785 104 98.9 251 108 100 7,799 102 99.1 107
EBOV 104 98.9 3,019 104 97.9 643 105 99.9 5,010 102 83.6 7
EV-D68 104 99.9 6,644 104 99.8 4,816 106 99.9 6,911 102 91.8 64
CVV-S 104 100 6,197 104 100 2,364 107 100 7,332 102 99.8 49
CVV-M 104 100 7,603 104 100 1,048 107 100 7,798 102 100 23
CVV-L 104 100 2,409 104 100 242 107 100 7,735 102 93.4 4
FLUAV -1 104 100 7,818 104 100 7,633 105 100 7,892 102 100 238
FLUAV -2 104 100 7,904 104 100 7,741 105 100 7,902 102 100 575
FLUAV -3 104 100 7,792 104 100 7,658 105 100 7,906 102 100 276
FLUAV -4 104 100 7,800 104 100 7,584 105 100 7,799 102 100 594
FLUAV -5 104 100 7,747 104 100 7,605 105 100 7,746 102 100 352
FLUAV -6 104 100 7,721 104 100 7,560 105 100 7,721 102 100 358
FLUAV -7 104 100 7,355 104 100 7,100 105 100 7,711 102 100 251
FLUAV -8 104 100 7,367 104 100 7,360 105 100 7,367 102 100 397
a

qPCR quantitated nucleic acid extracts representing seven different viruses were used to spike a background of human blood nucleic acid at levels of approximately 104 copies/100 ng, 102 copies/100 ng, and 105 to 108 copies/100 ng. Individual sequence libraries were prepared using 21 different indexes for bar coding. Libraries were mixed for capture hybridization into a 7-plex mix (libraries prepared from 104 loads) and the complete 21-plex mix.