FIG 2 .
Effect of apt deletion. (A) Relative counts of CFU recovered on MH agar from OD-equivalent cultures of the parental wild-type and Δapt::aprr strains. Mean results with SEM from six independent experiments are shown: **, P ≤ 0.01; ns, not significant. (B) Bright-field (differential interference contrast [DIC]) and fluorescence microscopy examining morphology and integrity of wild-type and Δapt::aprr strains. Fluorescence microscopy samples were costained with propidium iodide for DNA (red) and annexin V conjugate for permeabilization/phosphatidylserine (blue). Representative fields of view from two independent experiments are shown.