FIG 4 .

Detection of major R. bromii amylases by zymogram analysis and sequencing. (a) Zymogram showing activity of amylases against RS3 for R. bromii L2-63 cells grown for 24 h on 0.2% fructose or 0.2% RS3. Values on the left correspond to the molecular masses determined by staining the gel with Coomassie blue, prior to staining the gel with iodine to visualize clear zones of amylase activity. sup, supernatant proteins; cell, cell-associated proteins. Bands a to f, visible active bands. (b) Identification of amylolytic enzymes from excised bands by LC-MS/MS. In addition, a homologue of a Cna (collagen adhesion)-type protein was detected in band a, a hypothetical protein (RBR_05030) in band b, RNA polymerase subunit B in band d, and a hypothetical protein (RBR_07100) in band e.