FIG 6 .

Identification of native R. bromii proteins that interact with overexpressed Amy4-Coh1. Cell-associated or cell-free supernatant proteins of R. bromii L2-63 grown with boiled Novelose RS3 starch as the energy source were incubated with His6-tagged Coh1 at 37°C as described in Materials and Methods. Proteins binding to Coh1 were recovered and separated by SDS-PAGE, followed by proteomic analysis of individual bands. Protein identifiers (ID), molecular-mass values, and conserved modules for identified proteins are shown on the right of the figure. Numbers on the left indicate sizes and positions of molecular mass markers. SP, signal peptide; Amy, amylase; Coh, cohesin; Doc, dockerin; Anc, cell wall surface anchor; CBM, carbohydrate-binding module; Tri-tyrosine, triple-tyrosine motif.