TABLE 4 .
Interactions of recombinant R. bromii dockerin and cohesin modules determined by a microarray approacha
| Dockerin | Interaction |
Associated domain(s) | |||
|---|---|---|---|---|---|
| Coh1 (Amy4) | Coh2 (Sca2) | Coh3 (Sca3) | Coh4 (Sca4) | ||
| Amy9 | +++++ | +++ | +++++ | +++++ | GH13 |
| CBL16032.1 | +++ | ++ | +++++ | +++++ | Peptidase |
| CBL14720.1 | +++++ | +++ | +++++ | +++++ | 2× LRR |
| Amy4 | ++++ | +++ | +++++ | +++++ | GH13, Coh1 |
| Amy10 | +++++ | + | +++++ | +++++ | GH13, CBM26, CBM48 |
| CBL14834.1 | +++++ | +++ | +++++ | +++++ | |
| CBL14836.1 | − | − | − | + | Cysteine protease |
| CBL15647.1 | − | − | − | − | |
| Amy12 | − | − | ++ | +++++ | GH13, CBM48 |
| CBL15625.1 | − | − | − | − | |
| CBL16049.1 | − | − | − | − | |
a
Proteins were expressed as recombinant products (CBM fusions [cohesins] and XYN fusions [dockerins]) in E. coli (see Materials and Methods). LRR, = leucine-rich repeat. +++++, very strong; ++++, strong; +++, moderate; ++, weak; +, very weak; −, none.