TABLE 2 .
Primers used for construction of isoallelic mutant strains
| Primer | Sequence | Sequence underlined |
|---|---|---|
| ngapro-1 | CGCGTGGATCCCCGTGCTATCTTGTTGTCTATGGGT | BamHI |
| ngapro-V1A | TAAGTAAACTATACATTAGCAAATAGTTTTTGTCAATGCGA | SNPs to introduce |
| ngapro-V1B | TCGCATTGACAAAAACTATTTGCTAATGTATAGTTTACTTA | SNPs to introduce |
| ngapro-4 | CGCGTGGATCCTTTATCAATCTCAATGTGATGCGGT | BamHI site |
| ngapro-V3A | TAAGTAAACTATACATTAACAACTAGTTTTTGTCAATGCGA | SNPs to introduce |
| ngapro-V3B | TCGCATTGACAAAAACTAGTTGTTAATGTATAGTTTACTTA | SNPs to introduce |
| ngapro-seq | CGTGTTTACAAACCAATGGATGACT | |
| hasABCdel-1 | CGCGTGGATCCCTTTAGGTTGATACTGGGCGATGGA | BamHI site |
| hasABCdel-2 | TGGTCCTCGATAGAGCGTTTAGCTTTATTTTAGCAAACTTTTCAAATAGT | |
| hasABCdel-3 | ACTATTTGAAAAGTTTGCTAAAATAAAGCTAAACGCTCTATCGAGGACCA | |
| hasABCdel-4 | CGCGTGGATCCAATCCAAATTTATCCCCAACATCGT | BamHI site |
| 11027has-1 | CGCGTGGATCCCTTTAGGTTGATACTGGGCGATGGA | BamHI site |
| 11027has-2 | CGCGTGGATCCTCTACGGTTAAAAAAACGTCAGCGT | BamHI site |
| hasA−1 | CCGCGTGGATCCAATAAAGGAAAACGCCATGCTCAAG | BamHI site |
| hasA−2 | CCGCGTGGATCCATCATCCCCAATGCTAACAGGTAAA | BamHI site |