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. 2015 Sep 29;6(5):e01425-15. doi: 10.1128/mBio.01425-15

FIG 6 .

FIG 6 

The C. neoformans fnx1Δ mutant strain is deficient in invading the brain parenchyma. (A) Dorsal-viewpoint images taken from a z-axis stack of a 5-dpi Tg(kdrl:EGFPs843) larva infected with an mCherry-expressing C. neoformans CMO18 strain by caudal vein injection (left). Shown is a z-axis projection with a lightened box indicating a magnified region of interest (shown to the right) of 2× digital magnification slices at ventricle (60-µm) and brain tissue (120-µm) depths of focus. Blue arrows indicate C. neoformans cells located in the brain ventricle, red arrows indicate C. neoformans cells located in the brain parenchyma, and yellow arrows indicate C. neoformans cells located in the cranial vessels. Distances indicate the depth of image plane from the dorsal edge of the head. The full stack is available as Movie S8 in the supplemental material. (B) Dorsal-viewpoint images taken from a z-axis stack of a 5-dpi Tg(kdrl:EGFPs843) larva infected with an mCherry-expressing C. neoformans fnx1Δ mutant by caudal vein injection (left). Shown is a z axis projection with a lightened box indicating the magnified region of interest (shown to the right) of 2× digital magnification slices at ventricle (60-µm) and brain tissue (120-µm) depths of focus. Blue arrows indicate C. neoformans cells located in the brain ventricle. Distances indicate depths of the image planes from the dorsal edge of the head. The full stack is available as Movie S9 in the supplemental material. (C) Quantification of head ventricle C. neoformans colonization in 4-dpi larvae infected with the C. neoformans fnx1Δ mutant by caudal vein injection. Fisher’s exact test. Data are pooled from 3 replicates each with 10 to 20 larvae per genotype. (D) Quantification of cranial blood vessel C. neoformans colonization in 5-dpi larvae infected with the C. neoformans fnx1Δ mutant by caudal vein injection. Fisher’s exact test. Data are pooled from 4 replicates each with 10 to 20 larvae per genotype. (E) Quantification of brain tissue invasion in 5-dpi larva infected with the C. neoformans fnx1Δ mutant by caudal vein injection. Fisher’s exact test. Data are pooled from 3 replicates each with 10 to 20 larvae per genotype.