Table 1.
Protein levels of markers of inhibition and excitation pathways for WT and mBACtgDyrk1a (TG) in cortex, hippocampus, and cerebellum following treatment with EGCG-containing POL60 extract.
| Inhibitors Comparison Markers | POL 60 -EGCG 67.5 mg/kg | POL 60 -EGCG 67.5 mg/kg | POL 60 -EGCG 67.5 mg/kg | ||||||
|---|---|---|---|---|---|---|---|---|---|
| TG/WT | TG*/WT | TG*/TG | TG/WT | TG*/WT | TG*/TG | TG/WT | TG*/WT | TG*/TG | |
| CTX | HPC | CRB | |||||||
| DYRK1A | 162.2 ± 3.3 | 174.3 ± 5.4 | 174.3 ± 5.4 | 199.8 ± 9.2 | 208.8 ± 17 | 208.8 ± 17 | 208.7 ± 18.4 | 145.2 ± 11.8 | 145.2 ± 11.8 |
| p< 0.0001 | p< 0.0001 | p= 0.07 | p< 0.0001 | p< 0.0001 | p= 0.64 | p< 0.0001 | p = 0.004 | p = 0.01 | |
| GAD67 | 131.9 ± 6.6 | 108.1 ± 7.1 | 108.1 ± 7.1 | 142.2 ± 5.4 | 109.8 ± 8.4 | 109.8 ± 8.4 | 155.8 ± 8.9 | 117.6 ± 10.8 | 117.6 ± 10.8 |
| p < 0.0006 | p = 0.35 | p = 0.02 | p = 0.01 | p = 0.38 | p = 0.004 | p = 0.003 | p = 0.4 | p = 0.01 | |
| GAD65 | 136.4 ± 4 | 112.0 ± 5.1 | 112.0 ± 5.1 | 121.4 ± 2 | 112.7 ± 2.6 | 112.7 ± 2.6 | 145.8 ± 9.0 | 147.3 ± 5.4 | 147.3 ± 5.4 |
| p = 0.0001 | p = 0.07 | p = 0.0015 | p < 0.0001 | p = 0.001 | p = 0.01 | p = 0.001 | p < 0.0001 | p = 0.8 | |
| VGAT | 123.6 ± 2.9 | 105.3 ± 4 | 105.3 ± 4 | 125.3 ± 4.4 | 99.46 ± 9.2 | 99.46 ± 9.2 | 115.5 ± 3.5 | 106.2 ± 2.6 | 106.2 ± 2.6 |
| p < 0.0001 | p = 0.21 | p = 0.002 | p = 0.0003 | p = 0.9 | p = 0.01 | p = 0.0004 | p = 0.07 | p = 0.05 | |
| GLUR1 | 89.70 ± 2.9 | 105.8 ± 4.9 | 105.8 ± 4.9 | 93.71 ± 2.4 | 93.44 ± 3.5 | 93.44 ± 3.5 | 67.25 ± 7.0 | 66.55 ± 4.09 | 66.55 ± 4.09 |
| p = 0.01 | p = 0.35 | p = 0.01 | p = 0.1 | p = 0.15 | p = 0.9 | p = 0.001 | p < 0.0001 | p = 0.9 | |
| GLUR2 | 94.93 ± 2 | 86.89 ± 2.6 | 86.89 ± 2.6 | 83.63 ± 1.9 | 95.20 ± 2.7 | 95.20 ± 2.7 | 63.61 ± 2.4 | 86.44 ± 3.1 | 86.44 ± 3.1 |
| p = 0.12 | p = 0.001 | p = 0.02 | p = 0.0001 | p = 0.23 | p = 0.002 | p < 0.0001 | p = 0.01 | p < 0.0001 | |
| NR1 | 92.60 ± 3 | 92.16 ± 5.4 | 92.16 ± 5.4 | 88.38 ± 2.2 | 107.8 ± 7.2 | 107.8 ± 7.2 | 96.90 ± 3.7 | 94.35 ± 2.1 | 94.35 ± 2.1 |
| p = 0.09 | p = 0.2 | p = 0.9 | p = 0.02 | p = 0.33 | p = 0.01 | p = 0.7 | p = 0.45 | p = 0.55 | |
| NR2A | 87.47 ± 2.1 | 101.4 ± 9.3 | 101.4 ± 9.3 | 81.10 ± 1.9 | 100.4 ± 7.0 | 100.4 ± 7.0 | 72.12 ± 4.7 | 63.69 ± 3.4 | 63.69 ± 3.4 |
| p = 0.001 | p = 0.9 | p = 0.1 | p = 0.001 | p = 0.9 | p = 0.01 | p = 0.002 | p = 0.0001 | p = 0.17 | |
| VGLUT1 | 84.08 ± 1.8 | 107.8 ± 4.4 | 107.8 ± 4.4 | 106.3 ± 1.9 | 106.3 ± 2.8 | 106.3 ± 2.8 | 81.10 ± 4.5 | 78.04 ± 4.6 | 78.04 ± 4.6 |
| p = 0.0001 | p = 0.17 | p = 0.0001 | p = 0.04 | p = 0.1 | p = 0.9 | p = 0.01 | p = 0.005 | p = 0.6 | |
| VGAT/VGLUT1 | 149.7 ± 4.5 | 98.33 ± 7.3 | 98.33 ± 7.3 | 115.5 ± 4.8 | 92.84 ± 7.4 | 92.84 ± 7.4 | 136.7 ± 9.8 | 142.7 ± 9.3 | 142.7 ± 9.3 |
| p < 0.0001 | p = 0.75 | p = 0.0001 | p = 0.02 | p = 0.36 | p = 0.02 | p = 0.008 | p = 0.002 | p = 0.6 | |
| pCAMKII/CAMKII | 86.32 ± 2.8 | 72.58 ± 4.5 | 72.58 ± 4.5 | 79.32 ± 3.4 | 99.12 ± 8.1 | 99.12 ± 8.1 | 89.30 ± 5.5 | 93.70 ± 3.8 | 93.70 ± 3.8 |
| p = 0.02 | p = 0.0005 | p = 0.02 | p = 0.0004 | p = 0.9 | p = 0.04 | p = 0.08 | p = 0.5 | p < = 0.1 | |
Protein levels relative to the levels detected in wild-type animals (WT: 100). Mean expression relative to WT level ± standard error of the mean (SEM), P-values for the t-test comparing transgenic (TG) vs. wild-type (WT) with treated transgenic (TG*). (n = 10 for each genotype and for each treatment) CTX, cortex; HPC, Hippocampus; CRB, cerebellum. (Red indicates an increase, green a decrease, and blue no variation; changes were labeled for tendency toward significance with p ≤ 0.1: pink for tendancy toward increase and pale green for tendancy toward decrease).