Table 3.
Protein levels of markers of inhibition and excitation pathways for WT and mBACtgDyrk1a (Tg) in cortex following treatment with 3 doses of MGTE extract.
| Inhibitors Comparison Markers | MGTE- EGCG I- 10 mg/kg | MGTE- EGCG II- 60 mg/kg | MGTE- EGCGIII- 360 mg/kg | ||||
|---|---|---|---|---|---|---|---|
| TG/WT | TG*/WT | TG*/TG | TG*/WT | TG*/TG | TG*/WT | TG*/TG | |
| Cortex | |||||||
| DYRK1A | 162.2 ± 3.3 | 139.1 ± 7.9 | 139.1 ± 7.9 | 148.2 ± 8.0 | 148.2 ± 8.0 | 193.4 ± 14.0 | 193.4 ± 14.0 |
| p < 0.0001 | p < 0.0001 | p = 0.01 | p < 0.0001 | p = 0.1 | p < 0.0001 | p = 0.02 | |
| GAD67 | 131.9 ± 6.6 | 133.7 ± 10.8 | 133.7 ± 10.8 | 131.2 ± 5.7 | 131.2 ± 5.7 | 159.5 ± 15.6 | 159.5 ± 15.6 |
| p = 0.0006 | p = 0.0007 | p = 0.8 | p = 0.0003 | p = 0.9 | p < 0.0001 | p = 0.08 | |
| GAD65 | 136.4 ± 4 | 114.3 ± 7.0 | 114.3 ± 7.0 | 115.2 ± 6.4 | 115.2 ± 6.4 | 131.3 ± 8.9 | 131.3 ± 8.9 |
| p < 0.0001 | p = 0.04 | p = 0.01 | p = 0.05 | p = 0.01 | p = 0.002 | p = 0.56 | |
| VGAT | 123.6 ± 2.9 | 99.98 ± 5.3 | 99.98 ± 5.3 | 94.53 ± 3.7 | 94.53 ± 3.7 | 102.6 ± 3.7 | 102.6 ± 3.7 |
| p < 0.0001 | p = 0.9 | p = 0.0009 | p = 0.35 | p < 0.0001 | p = 0.7 | p = 0.0004 | |
| NR1 | 92.60 ± 3 | 99.53 ± 5.3 | 99.53 ± 5.3 | 91.98 ± 4.9 | 91.98 ± 4.9 | 85.50 ± 3.3 | 85.50 ± 3.3 |
| p = 0.09 | p = 0.87 | p = 0.1 | p = 0.1 | p = 0.9 | p = 0.006 | p = 0.1 | |
| NR2A | 87.47 ± 2.1 | 101.8 ± 4.5 | 101.8 ± 4.5 | 98.00 ± 2.6 | 98.00 ± 2.6 | 90.54 ± 2.6 | 90.54 ± 2.6 |
| p = 0.001 | p = 0.8 | p = 0.007 | p = 0.6 | p = 0.006 | p = 0.01 | p = 0.37 | |
| GLUR1 | 89.70 ± 2.9 | 94.90 ± 4.9 | 94.90 ± 4.9 | 106.7 ± 4.5 | 106.7 ± 4.5 | 111.3 ± 6 | 111.3 ± 6 |
| p = 0.01 | p = 0.39 | p = 0.36 | p = 0.25 | p = 0.006 | p = 0.15 | p = 0.005 | |
| GLUR2 | 94.93 ± 2 | 91.06 ± 2.4 | 91.06 ± 2.4 | 98.26 ± 4.2 | 98.26 ± 4.2 | 88.80 ± 1.9 | 88.80 ± 1.9 |
| p = 0.1 | p = 0.12 | p = 0.24 | p = 0.8 | p = 0.5 | p = 0.008 | p = 0.05 | |
| VGLUT1 | 84.08 ± 1.8 | 96.27 ± 3.6 | 96.27 ± 3.6 | 95.16 ± 3 | 95.16 ± 3 | 101.2 ± 5.9 | 101.2 ± 5.9 |
| p = 0.0001 | p = 0.34 | p = 0.005 | p = 0.19 | p = 0.005 | p = 0.8 | p = 0.006 | |
| VGAT/VGLUT1 | 149.7 ± 4.5 | 118.7 ± 2.1 | 118.7 ± 2.1 | 110.0 ± 4.9 | 110.0 ± 4.9 | 115.8 ± 7.3 | 115.8 ± 7.3 |
| p < 0.0001 | p = 0.02 | p = 0.02 | p = 0.22 | p = 0.0001 | p = 0.08 | p = 0.01 | |
| pCAMKII/CAMKII | 86.32 ± 2.8 | 80.14 ± 11.7 | 80.14 ± 11.7 | 52.77 ± 5.3 | 52.77 ± 5.3 | 102.1 ± 11.8 | 102.1 ± 11.8 |
| p = 0.01 | p = 0.08 | p = 0.48 | p < 0.0001 | p < 0.0001 | p = 0.79 | p = 0.08 | |
Protein levels relative to the levels detected in wild-type animals (WT: 100). Mean expression relative to WT level ± standard error of the mean (SEM), P-values for the t-test comparing transgenic (TG) vs. wild-type (WT) with treated transgenic (TG*). (n = 10). (Red indicates an increase, green a decrease, and blue no variation; changes were labeled for tendency toward significance with p ≤ 0.1: pink for tendancy toward increase and pale green for tendancy toward decrease).