Table 4.
Protein levels of markers of inhibition and excitation pathways for WT and mBACtgDyrk1a (TG) in hippocampus following treatment with 3 doses of MGTE extract.
| Inhibitors Comparison Markers | MGTE-EGCG I- 10 mg/kg | MGTE-EGCG II- 60 mg/kg | MGTE-EGCGIII- 360 mg/kg | ||||
|---|---|---|---|---|---|---|---|
| TG/WT | TG*/WT | TG*/TG | TG*/WT | TG*/TG | TG*/WT | TG*/TG | |
| Hippocampus | |||||||
| DYRK1A | 199.8 ± 9.2 | 164.9 ± 4.2 | 164.9 ± 4.2 | 129.5 ± 5.2 | 129.5 ± 5.2 | 164.4 ± 3.7 | 164.4 ± 3.7 |
| p < 0.0001 | p < 0.0001 | p = 0.004 | p = 0.002 | p = 0.0001 | p < 0.0001 | p = 0.008 | |
| GAD67 | 142.2 ± 5.4 | 123.5 ± 8.3 | 123.5 ± 8.3 | 123.8 ± 5.1 | 123.8 ± 5.1 | 148.4 ± 8.6 | 148.4 ± 8.6 |
| p < 0.0001 | p = 0.01 | p = 0.07 | p = 0.001 | p = 0.02 | p < 0.0001 | p = 0.46 | |
| GAD65 | 121.4 ± 2 | 122.1 ± 5.4 | 122.1 ± 5.4 | 118.2 ± 4.8 | 118.2 ± 4.8 | 117.1 ± 4.4 | 117.1 ± 4.4 |
| p < 0.0001 | p < 0.0001 | p = 0.8 | p = 0.002 | p = 0.7 | p = 0.0006 | p = 0.7 | |
| VGAT | 125.3 ± 4.4 | 95.55 ± 7.2 | 95.55 ± 7.2 | 102.9 ± 5.3 | 102.9 ± 5.3 | 101.2 ± 5.4 | 101.2 ± 5.4 |
| p = 0.0003 | p = 0.47 | p = 0.002 | p = 0.57 | p = 0.005 | p = 0.8 | p = 0.003 | |
| GLUR1 | 93.71 ± 2.4 | 83.72 ± 2.4 | 83.72 ± 2.4 | 94.06 ± 6.0 | 94.06 ± 6.0 | 92.94 ± 2.5 | 92.94 ± 2.5 |
| p = 0.1 | p = 0.003 | p = 0.01 | p = 0.31 | p = 0.9 | p = 0.24 | p = 0.43 | |
| GLUR2 | 83.63 ± 1.9 | 90.28 ± 2.6 | 90.28 ± 2.6 | 99.42 ± 1.9 | 99.42 ± 1.9 | 90.49 ± 4.8 | 90.49 ± 4.8 |
| p = 0.0001 | p = 0.1 | p = 0.05 | p = 0.9 | p = 0.0001 | p = 0.15 | p = 0.26 | |
| NR1 | 88.38 ± 2.2 | 108.3 ± 6.9 | 108.3 ± 6.9 | 113.2 ± 7.1 | 113.2 ± 7.1 | 133.9 ± 7.2 | 133.9 ± 7.2 |
| p = 0.02 | p = 0.31 | p = 0.01 | p = 0.1 | p = 0.005 | p = 0.0005 | p < 0.0001 | |
| NR2A | 81.10 ± 1.9 | 82.25 ± 4.4 | 82.25 ± 4.4 | 121.8 ± 6.7 | 121.8 ± 6.7 | 110.8 ± 4.6 | 110.8 ± 4.6 |
| p = 0.001 | p = 0.01 | p = 0.78 | p = 0.02 | p < 0.0001 | p = 0.29 | p < 0.0001 | |
| VGLUT1 | 106.4 ± 3.1 | 98.46 ± 5.7 | 98.46 ± 5.7 | 93.32 ± 4.3 | 93.32 ± 4.3 | 98.60 ± 5.0 | 98.60 ± 5.0 |
| p = 0.05 | p = 0.61 | p = 0.23 | p = 0.05 | p = 0.02 | p = 0.7 | p = 0.24 | |
| VGAT/VGLUT1 | 115.5 ± 4.8 | 89.13 ± 4.8 | 89.13 ± 4.8 | 111.2 ± 3.9 | 111.2 ± 3.9 | 103.8 ± 5.8 | 103.8 ± 5.8 |
| p = 0.02 | p = 0.07 | p = 0.001 | p = 0.05 | p = 0.5 | p = 0.58 | p = 0.1 | |
| pCAMKII/CAMKII | 79.33 ± 3.3 | 75.63 ± 6.6 | 75.63 ± 6.6 | 99.77 ± 12.3 | 99.77 ± 12.3 | 96.93 ± 7.4 | 96.93 ± 7.4 |
| p = 0.0004 | p = 0.005 | p = 0.59 | p = 0.89 | p = 0.1 | p = 0.81 | p = 0.04 | |
Protein levels relative to the levels detected in wild-type animals (WT: 100). Mean expression relative to WT level ± standard error of the mean (SEM), P-values for the t-test comparing transgenic (TG) vs. wild-type (WT) with treated transgenic (TG*). (n = 10). (Red indicates an increase, green a decrease, and blue no variation; changes were labeled for tendency toward significance with p ≤ 0.1: pink for tendancy toward increase and pale green for tendancy toward decrease).