Table 5.
Protein levels of markers of inhibition and excitation pathways for WT and mBACtgDyrk1a (Tg) in cerebellum following treatment with 3 doses of MGTE extract.
| Treatment Markers | MGTE-EGCG I- 10 mg/kg | MGTE-EGCG II- 60 mg/kg | MGTE-EGCGIII- 360 mg/kg | ||||
|---|---|---|---|---|---|---|---|
| TG/WT | TG*/WT | TG*/TG | TG*/WT | TG*/TG | TG*/WT | TG*/TG | |
| Cerebellum | |||||||
| DYRK1A | 171.0 ± 9 | 136.5 ± 9.7 | 136.5 ± 9.7 | 139.4 ± 7.0 | 139.4 ± 7.0 | 155.6 ± 13.9 | 155.6 ± 13.9 |
| p < 0.0001 | p = 0.003 | p = 0.01 | p = 0.0002 | p = 0.01 | p < 0.0001 | p = 0.35 | |
| GAD67 | 159.8 ± 6.7 | 150.6 ± 13.0 | 150.6 ± 13.0 | 181.4 ± 10.9 | 181.4 ± 10.9 | 188.6 ± 17.3 | 188.6 ± 17.3 |
| p = 0.0001 | p = 0.002 | p = 0.5 | p = 0.0001 | p = 0.1 | p < 0.0001 | p = 0.1 | |
| GAD65 | 139.6 ± 5.1 | 138.1 ± 5.6 | 138.1 ± 5.6 | 148.2 ± 9.05 | 148.2 ± 9.05 | 168.2 ± 13.6 | 168.2 ± 13.6 |
| p = 0.0001 | p < 0.0001 | p = 0.85 | p = 0.0002 | p = 0.41 | p = 0.0001 | p = 0.04 | |
| VGAT | 122.6 ± 3.2 | 136.6 ± 4.0 | 136.6 ± 4.0 | 133.6 ± 3.6 | 133.6 ± 3.6 | 135.3 ± 3.9 | 135.3 ± 3.9 |
| p < 0.0001 | p < 0.0001 | p = 0.68 | p < 0.0001 | p = 0.04 | p < 0.0001 | p = 0.02 | |
| GLUR1 | 82.87 ± 2.6 | 98.01 ± 5.3 | 98.01 ± 5.3 | 92.62 ± 5.3 | 92.62 ± 5.3 | 100.7 ± 6.2 | 100.7 ± 6.2 |
| p = 0.0005 | p = 0.74 | p = 0.01 | p = 0.21 | p = 0.1 | p = 0.91 | p = 0.01 | |
| GLUR2 | 87.94 ± 2.7 | 103.6 ± 3.6 | 103.6 ± 3.6 | 107.2 ± 2.8 | 107.2 ± 2.8 | 111.7 ± 4.7 | 111.7 ± 4.7 |
| p = 0.009 | p = 0.48 | p = 0.003 | p = 0.09 | p = 0.0001 | p = 0.04 | p = 0.0005 | |
| NR1 | 98.84 ± 1.6 | 90.71 ± 2.6 | 90.71 ± 2.6 | 94.11 ± 4.1 | 94.11 ± 4.1 | 94.18 ± 3.5 | 94.18 ± 3.5 |
| p = 0.7 | p = 0.08 | p = 0.01 | p = 0.3 | p = 0.28 | p = 0.2 | ||
| NR2A | 85.2 ± 2.9 | 94.08 ± 5.6 | 94.08 ± 5.6 | 89.99 ± 3.6 | 89.99 ± 3.6 | 91.1 ± 3.8 | 91.15 ± 3.8 |
| p = 0.001 | p = 0.4 | p = 0.15 | p = 0.04 | p = 0.36 | p = 0.07 | p = 0.31 | |
| VGLUT1 | 84.5 ± 2 | 83.82 ± 2.7 | 83.82 ± 2.7 | 81.72 ± 3.0 | 81.72 ± 3.0 | 77.67 ± 2.1 | 77.67 ± 2.1 |
| p < 0.0001 | p = 0.0005 | p = 0.66 | p = 0.0001 | p < 0.33 | p < 0.0001 | p = 0.07 | |
| VGAT/VGLUT1 | 141.6 ± 5.1 | 161.1 ± 3.7 | 161.1 ± 3.7 | 162.0 ± 8.4 | 162.0 ± 8.4 | 173.1 ± 7.8 | 173.1 ± 7.8 |
| p < 0.0001 | p < 0.0001 | p = 0.008 | p < 0.0001 | p = 0.06 | p < 0.0001 | p = 0.004 | |
Protein levels relative to the levels detected in wild-type animals (WT: 100). Mean expression relative to WT level ± standard error of the mean (SEM), P-values for the t-test comparing transgenic (TG) vs. wild-type (WT) with treated transgenic (TG*). (n = 10). (Red indicates an increase, green a decrease, and blue no variation; changes were labeled for tendency toward significance with p ≤ 0.1: pink for tendancy toward increase and pale green for tendancy toward decrease).