Table 6.
Protein levels of markers of inhibition and excitation pathways for WT and mBACtgDyrk1a (Tg) in cortex and hippocampus following treatment with caffeine.
| Inhibitors Comparison Markers | Caffeine | Caffeine | ||||
|---|---|---|---|---|---|---|
| TG/WT | TG*/WT | TG*/TG | TG/WT | TG*/WT | TG*/TG | |
| CTX | HPC | |||||
| DYRK1A | 162.2 ± 3.3 | 180.1 ± 8.1 | 180.1 ± 8.1 | 199.8 ± 9.2 | 149.4 ± 14 | 149.4 ± 14 |
| p < 0.0003 | p < 0.0001 | p = 0.8 | p < 0.0001 | p < 0.0001 | p = 0.006 | |
| GAD67 | 131.9 ± 6.6 | 110.3 ± 7.8 | 110.3 ± 7 | 142.2 ± 5.4 | 103.7 ± 9.2 | 103.7 ± 9.2 |
| p = 0.0006 | p = 0.22 | p = 0.05 | p < 0.0001 | p = 0.8 | p = 0.001 | |
| VGAT | 123.6 ± 2.9 | 109.3 ± 7.9 | 109.3 ± 7.9 | 125.3 ± 4.4 | 99.46 ± 9.2 | 99.46 ± 9.2 |
| p < 0.0001 | p = 0.2 | p = 0.07 | p = 0.0003 | p = 0.9 | p = 0.008 | |
| GLUR1 | 89.70 ± 2.9 | 85.94 ± 2.8 | 85.94 ± 2.8 | 93.71 ± 2.4 | 93.44 ± 3.5 | 93.44 ± 3.5 |
| p = 0.01 | p = 0.002 | p = 0.39 | p = 0.1 | p = 0.1 | p = 0.9 | |
| GLUR2 | 94.93 ± 2 | 96.86 ± 5 | 96.86 ± 5 | 83.63 ± 1.9 | 85.94 ± 2.8 | 85.94 ± 2.8 |
| p = 0.1 | p = 0.58 | p = 0.9 | p = 0.0001 | p = 0.003 | p = 0.5 | |
| NR1 | 92.60 ± 3 | 85.18 ± 3.8 | 85.18 ± 3.8 | 88.38 ± 2.2 | 68.20 ± 5.6 | 68.20 ± 5.6 |
| p = 0.09 | p = 0.01 | p = 0.1 | p = 0.02 | p = 0.0004 | p = 0.001 | |
| NR2A | 87.47 ± 2.1 | 69.23 ± 4 | 69.23 ± 4 | 81.10 ± 1.9 | 76.56 ± 7.3 | 76.56 ± 7.3 |
| p = 0.001 | p < 0.0001 | p = 0.0005 | p = 0.001 | p = 0.01 | p = 0.49 | |
| VGLUT1 | 84.08 ± 1.8 | 91.54 ± 2.7 | 91.54 ± 2.7 | 106.3 ± 1.9 | 98.12 ± 4.4 | 98.12 ± 4.4 |
| p < 0.0001 | p = 0.01 | p = 0.03 | p = 0.04 | p = 0.5 | p = 0.15 | |
| VGAT/VGLUT1 | 149.7 ± 4.5 | 125.8 ± 9.2 | 125.8 ± 9.2 | 115.5 ± 4.8 | 96.09 ± 7 | 96.09 ± 7 |
| p < 0.0001 | p = 0.01 | p = 0.02 | p = 0.02 | p = 0.56 | p = 0.03 | |
| pCAMKII/CAMKII | 86.35 ± 3.3 | 100.7 ± 8.6 | 100.7 ± 8.6 | 79.32 ± 3.4 | 115.9 ± 5.5 | 115.9 ± 5.5 |
| p = 0.02 | p = 0.9 | p = 0.1 | p = 0.0004 | p = 0.01 | p < 0.0001 | |
Protein levels relative to the levels detected in wild-type animals (WT: 100). Mean expression relative to WT level ± standard error of the mean (SEM), P-values for the t-test comparing transgenic (TG) vs. wild-type (WT) with treated transgenic (TG*). (n = 10) CTX, cortex; HPC, Hippocampus; CRB, cerebellum. (Red indicates an increase, green a decrease, and blue no variation; changes were labeled for tendency toward significance with p ≤ 0.1: pink for tendancy toward increase and pale green for tendancy toward decrease).