Figure 2.

SC-43 triggers apoptosis via p-STAT3^Tyr705 downregulation. (A) Western blotting of p-STAT3^Tyr705, the cleaved fragments of PARP, and the survival markers (cyclin D1, Mcl-1, and survivin) in cells 24 hours after treatment with dose escalation of SC-43. β-Actin was used as a loading control. The cleaved fragments of PARP are indicated by arrows. (B) Overexpression of STAT3 in Hct-116 and Hct-15 cells rescued apoptosis induced by treatment with 5 μM SC-43 for 24 hours. Data from apoptosis assay are shown as mean ± SD of three independent experiments, made in triplicate. *P < .05.