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. 2015 Oct 19;17(9):742–754. doi: 10.1016/j.neo.2015.09.005

Figure 1.

Figure 1

SUMO proteins are upregulated and SENP1 protease is downregulated in NPM-ALK+ T-cell lymphoma. (A) WB studies show very low levels of expression of SUMO-1 and SUMO-2/3 proteins in normal human T lymphocytes. In contrast, overexpression of SUMO-2/3 protein is present in the five NPM-ALK+ T-cell lymphoma cell lines Karpas 299, SR-786, DEL, SUP-M2, and SU-DHL-1. In addition, increased expression of SUMO-1 protein is seen in majority of the lymphoma cell lines including Karpas 299, SR-786, and SU-DHL-1. Moreover, high levels of expression of the SENP1 protease are detected in the normal human T lymphocytes compared with much lower levels in the five lymphoma cell lines. The Jurkat and 786-O cells were used as positive and negative controls, respectively, for the expression of the SUMO proteins. Notably, Jurkat cells that express high levels of the SUMO proteins demonstrate much lower levels of SENP1 than the 786-O cells that lack the expression of SUMO proteins. β-Actin was used as a loading control. (B) Densitometry studies of SUMO and SENP1 proteins relative to β-actin support the findings of the WB. (C) WB performed on protein extracted from FFPE tissue sections collected from 15 patients’ lymphoma tumors. The patient samples were divided into two groups—1 to 7 (left panel) and 8 to 15 (right panel)—and lysates from normal human T lymphocytes (TL) were analyzed. It is important to notice that the quality of the FFPE tissue sections varied significantly, and therefore, β-actin showed unequal protein levels among these samples. Nonetheless, WB revealed a pattern of expression similar to the cell lines in which SUMO-2/3 was overexpressed in all patients, and SUMO-1 was upregulated in 9 of the 15 patients. In clear contrast, SENP1 was decreased in 13 of the 15 patients. β-Actin was used as a loading control. (D) Densitometry studies of the WB bands of SUMO-1, SUMO-2/3, and SENP1 proteins relative to β-actin bands are shown. Despite the variable levels of β-actin in the lysates from patients’ FFPE tumors, densitometry supported the general increase in SUMO proteins and decrease in SENP1 protease in these samples.