Figure 4.

LPA₂ is the major LPA receptor subtype responsible for HK2 induction and glycolysis. Each of LPA_1 -3 receptors was knocked down by siRNA in Caov-3 cells or by lentivirus-transduced shRNA in OVCA-432 cells. (A) The cells were serum starved and treated with LPA (10 μM) or vehicle for 16 hours before immunoblotting analysis of HK2 protein. (B) Glycolysis of these cells was determined as in Figure 1 and the results were presented as fold increases relative to vehicle-treated control cells (defined as one fold). (C) LPA-induced increases in HK2 expression and glycolysis were reproduced in LPA₂-expressing MDA-231 breast cancer and HCT116 colon cancer cell lines.