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Weak affinity for DndE to DndC and IscS. (A) DndC interacts with DndE. After nickel affinity purification, proteins were separated by SDS-PAGE. The identity of DndC was confirmed by mass spectrometry. (B) IscS interacts with DndE. Lysate for construct over-expressing DndE-his6 was mixed with that for construct over-expressing IscS-strep tag and purified by nickel affinity column.
