Table 3.
OTUs exclusively associated with P. carnosus diseased tissues.
| OTU | # | RDP 16S rRNA reference sequences database v9 | Closest Blastn match with NCBI GenBank nt database | |||||
|---|---|---|---|---|---|---|---|---|
| Phylum | Class | Order | RDP classified taxon (80% confidence threshold) | Accession no. | % Similarity | Source | ||
| 763 | 36 | Acidobacteria | Acidobacteria | Acidobacteria | Acidobacteria (Genus Gp10) | FJ205217 | 91 | Uncultured Acidobacteria bacterium (hydrothermal field sediment) |
| 783 | 6 | Actinobacteria | Actinobacteria | Acidimicrobiales | Acidimicrobineae (unclassified) | KF886137 | 97 | Uncultured bacterium (hydrothermal field sediment) |
| 336 | 34 | Actinobacteria | Unclassified | Unclassified | Unclassified Actinobacteria | EU491785 | 95 | Uncultured bacterium (Ocean crust) |
| 1385 | 101 | Bacteroidetes | Cytophagia | Cytophagales | Flammeovirgaceae (unclassified) | GU369880 | 99 | Uncultured Bacteroidetes bacterium (marine microbial mat) |
| 2093 | 6 | Bacteroidetes | Flavobacteria | Flavobacteriales | Flavobacteriaceae (unclassified) | GQ412799 | 98 | Uncultured bacterium (coral Porites cylindrica) |
| 3513 | 62 | Bacteroidetes | Flavobacteria | Flavobacteriales | Flavobacteriaceae (Genus Algibacter) | JN830951 | 98 | Uncultured bacterium (seafloor sediment) |
| 2567 | 22 | Bacteroidetes | Flavobacteria | Flavobacteriales | Flavobacteriaceae (Genus Maritimimonas) | JX527388 | 95 | Uncultured Flavobacteriaceae bacterium (seawater) |
| 2382 | 5 | Cyanobacteria | Cyanobacteria | Cyanobacteria | Family IV (Genus GpIV) | JQ727114 | 99 | Uncultured bacterium (marine biofilm) |
| 2705 | 24 | Cyanobacteria | Cyanobacteria | Cyanobacteria | Family II (Genus GpIIa) | GU061669 | 100 | Uncultured bacterium (marine sediment) |
| 3175 | 36 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Hyphomicrobiaceae (Genus Filomicrobium) | HQ601714 | 99 | Uncultured bacterium (marine biofilm) |
| 1986 | 48 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Phyllobacteriaceae (Genus Hoeflea) | EF123312 | 99 | Uncultured alphaproteobacterium (black band disease in coral Siderastrea siderea) |
| 1071 | 263 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Phyllobacteriaceae (unclassified) | JF272168 | 98 | Uncultured bacterium (marine biofilm) |
| 1706 | 16 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Rhizobiales incertae sedis (Genus Bauldia) | NR117251 | 99 | Bauldia litoralis strain |
| 2601 | 55 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Rhizobiales (unclassified) | JF268389 | 100 | Uncultured bacterium (deep-sea sediment) |
| 3389 | 24 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Rhodobiaceae (Genus Anderseniella) | EU346403 | 100 | Marine sponge bacterium (Haliclona (gellius) sp.) |
| 3528 | 29 | Proteobacteria | Alphaproteobacteria | Rhizobiales | Rhodobiaceae (Genus Anderseniella) | FJ930408 | 99 | Uncultured bacterium (coral-associated bacterial community) |
| 398 | 34 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (Genus Roseovarius) | NR044424 | 99 | Roseovarius aestuarii sp. from tidal flat |
| 1763 | 1531 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (Genus Roseovarius) | HQ242427 | 99 | Uncultured Rhodobacteraceae bacterium (seawater) |
| 2586 | 22 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (Genus Roseovarius) | EF123384 | 99 | Uncultured alphaproteobacterium (black band disease in coral Siderastrea siderea) |
| 3330 | 448 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (Genus Ruegeria) | KF179946 | 99 | Uncultured bacterium (white patch syndrome in coral Porites lutea) |
| 3536 | 47 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (unclassified) | DQ200606 | 97 | Uncultured alphaproteobacterium (coral Montastraea annularis) |
| 3086 | 35 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (unclassified) | JF835684 | 99 | Uncultured bacterium (bleached colonies of coral Siderastrea stellata) |
| 2625 | 1138 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (unclassified) | JX405520 | 98 | Uncultured marine bacterium (seawater) |
| 2816 | 92 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (unclassified) | GU066430 | 100 | Uncultured bacterium (marine biofilm) |
| 497 | 27 | Proteobacteria | Alphaproteobacteria | Rhodobacterales | Rhodobacteraceae (unclassified) | AB694457 | 98 | Uncultured bacterium (deep-sea sediment, depth 7111 m) |
| 1468 | 119 | Proteobacteria | Alphaproteobacteria | Rhodospirillales | Rhodospirillaceae (Genus Pelagibius) | GU118834 | 99 | Uncultured bacterium (coral-associated bacterial community) |
| 1644 | 107 | Proteobacteria | Alphaproteobacteria | Rhodospirillales | Rhodospirillaceae (unclassified) | JQ516560 | 99 | Uncultured Rhodospirillaceae bacterium (coral Montastraea faveolata) |
| 3098 | 52 | Proteobacteria | Alphaproteobacteria | Sphingomonadales | Sphingomonadales (unclassified) | EF491353 | 99 | Uncultured alphaproteobacterium (seawater) |
| 1880 | 16 | Proteobacteria | Alphaproteobacteria | Unclassified | Unclassified Alphaproteobacteria | HQ153947 | 99 | Uncultured bacterium (marine microbial mat) |
| 2325 | 357 | Proteobacteria | Alphaproteobacteria | Unclassified | Unclassified Alphaproteobacteria | FJ202551 | 99 | Uncultured bacterium (white plague disease in coral Montastraea faveolata) |
| 2641 | 24 | Proteobacteria | Deltaproteobacteria | Myxococcales | Myxococcales (unclassified) | JQ580213 | 95 | Uncultured deltaproteobacterium (oil-polluted subtital sediment) |
| 3476 | 38 | Proteobacteria | Deltaproteobacteria | Unclassified | Unclassified Deltaproteobacteria | AY592337 | 96 | Uncultured bacterium (Deep-sea mud volcano) |
| 588 | 64 | Proteobacteria | Gammaproteobacteria | Incertae sedis | Gammaproteobacteria incertae sedis (unclassified) | GU319278 | 98 | Uncultured marine bacterium (coral Acropora eurystoma) |
| 1277 | 11 | Proteobacteria | Gammaproteobacteria | Incertae sedis | Gammaproteobacteria incertae sedis (unclassified) | FJ203535 | 99 | Uncultured bacterium (white plague disease in coral Montastraea faveolata) |
| 2513 | 475 | Proteobacteria | Gammaproteobacteria | Incertae sedis | Gammaproteobacteria incertae sedis (Genus Arenicella) | EU290222 | 97 | Uncultured bacterium (marine sponge Tethya californiana) |
| 1891 | 261 | Proteobacteria | Gammaproteobacteria | Unclassified | Unclassified Gammaproteobacteria | FJ202524 | 99 | Uncultured bacterium (white plague disease in coral Montastraea faveolata) |
| 1917 | 8 | Proteobacteria | Gammaproteobacteria | Unclassified | Unclassified Gammaproteobacteria | JF261866 | 98 | Uncultured bacterium (marine biofilm) |
| 3469 | 13 | Proteobacteria | Gammaproteobacteria | Unclassified | Unclassified Gammaproteobacteria | AB694447 | 98 | Uncultured bacterium (seafloor sediment) |
| 689 | 160 | Proteobacteria | Gammaproteobacteria | Unclassified | Unclassified Gammaproteobacteria | GU118018 | 99 | Uncultured bacterium (coral-associated bacteria community) |
| 1015 | 24 | Proteobacteria | Gammaproteobacteria | Unclassified | Unclassified Gammaproteobacteria | GQ412875 | 99 | Uncultured bacterium (coral-associated bacterial community) |
| 1495 | 73 | Proteobacteria | Gammaproteobacteria | Unclassified | Unclassified Gammaproteobacteria | AM911359 | 97 | Uncultured bacterium (coral Lophelia pertusa) |
| 527 | 51 | Unclassified | Unclassified | Unclassified | Unclassified Bacteria | EF629794 | 98 | Uncultured deltaproteobacterium (sponge Ircinia strobilina) |
These 42 OTUs were observed in all four replicates of coral diseased tissues but not in any apparently healthy tissues of SGA-affected colonies. Taxonomic classification of each OTU is given as far as it could be determined at the 80% confidence threshold with the RDP classifier based on the 16S rRNA reference sequences database v9. The closest Blastn match of each OTU in the NCBI GenBank nt database is given with the accession number, similarity and source. OTU refers to the unique identity number assigned to each OTU and # indicates the number of sequences represented by that OTU. Text in bold indicate OTUs that affiliate with sequences from other coral-associated bacteria.